• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Culture chamber for competing microbiological tests by volatile compounds, comprising a first receptacle (1), a second receptacle (2), and a central part (3) between the first receptacle (1) and the second receptacle (2), wherein the receptacles (1, 2) comprise an outer wall (4) and a perimeter wall (5) perpendicular to the external wall (4), and wherein the central part (3) comprises an intermediate wall (6) with at least one hole (13), and two side walls (7) projecting from the perimeter of the intermediate wall (6) in opposite directions, such that the side walls (7) of the central part (3) surround the perimeter walls (5) of the receptacles (1, 2) and the perimeter walls (5) of the receptacles (1, 2) rest on the intermediate wall (6). (Machine-translation by Google Translate, not legally binding)
    公开号:ES2708899A1
    申请号:ES201830817
    申请日:2018-08-10
    公开日:2019-04-11
    发明作者:García Samuel Alvarez；Luelmo Pedro Antonio Casquero；Martín Santiago Gutiérrez；Prieto Sara Mayo；López Oscar González；Huerga Guzmán Carro；Villanueva Víctor Suárez；González Alvaro Rodríguez
    申请人:Universidad de Leon；
    IPC主号:
    专利说明:

    [0001] c Am a r a d e c u l t i o p a r a n e a n d m a r e s o f o r g e s
    [0002]
    [0003] d e s c r ip c io n
    [0004]
    [0005] Field of the invention
    [0006] The present invention relates to a culture chamber for competing microbiological tests by volatile compounds. The culture chamber of the invention is applicable in the field of microbiological analysis techniques, and more specifically that of culture plates or Petri dishes.
    [0007]
    [0008] Background of the invention
    [0009] The culture plates, also known as Petri dishes, are containers of various sizes (with diameters varying between 50 mm and 150 mm), generally shallow and circular, although they may have other shapes and / or sizes.
    [0010]
    [0011] Petri dishes are the fundamental element used in the cultivation of microorganisms in solid medium.
    [0012]
    [0013] Currently the most common materials in the manufacture of these plates are plastic materials, however there are glass Petri dishes or other materials.
    [0014]
    [0015] A Petri dish is composed of two basic elements: a base where the corresponding culture medium is located, and a cover that covers the previous one.
    [0016]
    [0017] The ways in which these two elements are adjusted or fit are varied, being able to remain loose with respect to each other or to be fixed between them by means of various anchoring elements (mechanical tightening or mechanical locking by means of rotation or hooking), avoiding in the latter case the Crop contamination by opening or accidental falls. In addition, the closure can be complete, thus generating a watertight space inside and avoiding or limiting the exchange of gases with the outside of the plate (unventilated plates), or there may be various projections or protuberances in one of the two elements that they generate a sufficient space between them so that there is a certain exchange or flow of gases between the inside and the outside of the plate (ventilated plates).
    [0018] For the cultivation of microorganisms, the management of gaseous flows and their applications is of vital importance, among which are:
    [0019] - provide the necessary oxygen concentration for aerobic microorganisms or limit it for anaerobes;
    [0020] - regulate the drying of the medium once it is deposited in the Petri dish, avoiding condensation of moisture in the case of ventilated plates or avoiding excessive drying in those long-duration cultures in the non-ventilated plates; Y
    [0021] - regulate the exit to the outside of volatile compounds produced by the microorganisms in culture.
    [0022]
    [0023] Competency tests between microorganisms have become essential tools for the in vitro characterization of strains with biological control capacity and for the detection of bioactive compounds produced by them. These experiments can be developed in solid medium (routinely in Petri dishes) or liquid (using other techniques and materials). In general, there are three types of in vitro tests of competition between microorganisms in solid medium:
    [0024] - experiments of direct competition, in which strains of microorganisms come into direct contact;
    [0025] - competition experiments through soluble compounds, in which strains of microorganisms are faced only with soluble compounds produced by other strains without direct physical contact between them; Y
    [0026] - Experiments of competition through volatile, in which strains of microorganisms face only volatile compounds produced by other strains without direct physical contact between them.
    [0027]
    [0028] In the scientific bibliography the following means have been described to perform this type of competition experiments through volatile compounds:
    [0029] - Petri dishes subdivided, are plates with one or more partitions that divide the surface of the same in several parts where microorganisms can be grown without coming into contact. These systems limit the growth space for each strain, preventing them from being individually cultured in the center of the plate and are not viable to measure the radial growth of the microorganism colonies. There is also risk of cross contamination, either through spores or because the mycelium is able to overcome the septum of separation.
    [0030] - introduction of open Petri dishes into larger containers.
    [0031] This system is complex, it involves an extra expense and makes handling and taking measurements very difficult during the period in which the experiment is carried out.
    [0032] - arrangement of two facing Petri dishes, where each of the strains is grown in the center of a Petri dish (routine) and then, after removing the caps from them, join the upper limits of both plates faced by some type of plastic adhesive. In this way both crops are confronted in a closed space (a kind of chamber formed by the volume of the two plates), one growing in the lower internal part (usually the volatiles producing microorganism) and another in the upper internal part of the body. camera (usually the microorganism on which the effect of the compounds is evaluated). This is one of the most used systems for the evaluation of the activity of the volatiles produced by fungi of biological control. Allows some manipulation of the material once the experiment has begun, take measurements of the growth of the colonies and take pictures of them.
    [0033]
    [0034] However, the option of having two Petri dishes facing each other poses a series of problems, all derived from the non-existence of a specific commercial device designed to carry out this type of experiments:
    [0035] - existence of cross contamination: by spores, mycelium growth on the walls of the plates (polluting the opposite crop) or by contact between the aerial parts of the mycelia. The average expert in microbiological analysis understands that these contaminations distort the results of the experiment. An intermediate membrane can be placed to avoid contamination, however the disposal of the same is complex and difficult to systematize given that there are no specific anchoring or securing structures.
    [0036] - difficulty in the facing arrangement of the plates since there are no Petri dishes designed for this function, so they do not fit properly and this makes the handling and the reproducibility of the experiment itself difficult, besides there is no way to ensure that the lace has been perfect or, on the contrary, there have been small spaces without contact between the edges of both plates.
    [0037] - impossibility of regulating the air intake or gas exchange with the outside, in these experiments with facing plates it is not possible to make use of these different dispositions due to the fact that the covers have to be discarded, and the edges of the bases must be united with some type of adhesive.
    [0038] - unnecessary waste of material when discarding the covers of the plates used for lack of use in this type of experiments.
    [0039]
    [0040] The present patent / utility model is aimed at solving the aforementioned problems generated in the development and development of competition experiments between microorganisms through volatile compounds. A novel culture chamber specially designed for the realization of these experiments is described
    [0041]
    [0042] Description of the invention
    [0043] The subject of the invention is a culture chamber for competing microbiological assays by volatile compounds comprising: a first receptacle, a second receptacle, and a centerpiece between the first receptacle and the second receptacle.
    [0044]
    [0045] The receptacles comprise an outer wall and a perimeter wall perpendicular to the external wall, and the central part comprises an intermediate wall with at least one hole, and two side walls projecting from the perimeter of the intermediate wall in opposite directions. In the plate object of the invention, the side walls of the central part surround the perimeter walls of the receptacles and the perimeter walls of the receptacles rest on the intermediate wall.
    [0046]
    [0047] In the culture chamber for competition microbiological tests by volatile compounds object of the invention, the middle wall of the central piece comprises a first face facing the first receptacle and a second face facing the second receptacle, where the first face and the second face face are smooth.
    [0048]
    [0049] In another embodiment of the culture chamber for competition microbiological tests by volatile compounds object of the invention, the central piece comprises an internal rim on at least one of the faces of the intermediate wall, such that the inner flange and the side wall form a housing for the end of the outer wall of a receptacle.
    [0050]
    [0051] In another embodiment of the culture chamber for competing microbiological tests by volatile compounds object of the invention, the intermediate wall comprises a plurality of tabs on the first face and / or on the second face where the outer wall of a receptacle rests.
    [0052]
    [0053] In another embodiment the culture chamber for competing microbiological assays by volatile compounds comprises a membrane or filter covering the hole in the middle wall of the centerpiece, where the membrane or filter is porous allowing the passage of air and volatile compounds, and blocking the passage of larger elements.
    [0054]
    [0055] The culture chamber for competing microbiological assays by volatile compounds object of the invention comprises a first flange located on the external wall of the first receptacle, and a second rim of slightly different diameter located on the external wall of the second receptacle, such that the walls external of a first receptacle and a second receptacle, the first flange fits into the second flange preventing relative movement between two stacked culture plates.
    [0056]
    [0057] In another embodiment, the culture chamber for competing microbiological assays by volatile compounds object of the invention comprises at least one partition in at least one receptacle, such that the partition divides the interior of said receptacle in independent parts.
    [0058]
    [0059] Brief description of the drawings
    [0060] To complement the description that will be made next and in order to help a better understanding of the characteristics of the invention, the descriptive specification of a set of drawings based on which the innovations will be more easily understood will be accompanied. advantages of the device object of the invention.
    [0061]
    [0062] Figure 1 shows an exploded view of the culture chamber object of the invention.
    [0063] Figure 2 shows a sectional view of the culture chamber object of the assembled invention.
    [0064]
    [0065] Figure 3 shows sections of two embodiments of the joining means between the two receptacles and the central piece and a third section showing the tabs to provide a cultivation chamber with aeration.
    [0066]
    [0067] Figure 4 shows in detail the central hole of the horizontal intermediate wall of the intermediate element, with a variant in which said hole is open, and another variant with said hole covered by a membrane or filter.
    [0068]
    [0069] Figure 5 shows two plates of stacked crops.
    [0070]
    [0071] The different numerical references that are reflected in the figures correspond to the following elements:
    [0072] 1. first receptacle,
    [0073] 2. second receptacle,
    [0074] 3. center piece,
    [0075] 4. external wall,
    [0076] 5. perimeter wall,
    [0077] 6. intermediate wall,
    [0078] 7. side wall,
    [0079] 8. first face,
    [0080] 9. second side,
    [0081] 10. inner flange,
    [0082] 11. accommodation,
    [0083] 12. eyelashes,
    [0084] 13. hole,
    [0085] 14. membrane or filter,
    [0086] 15. partition,
    [0087] 16. first flange, and
    [0088] 17. second flange.
    [0089] The culture chamber object of the invention comprises a first receptacle (1) and a second receptacle (2) and a central part (3) that is located between the first receptacle (1) and the second receptacle (2).
    [0090]
    [0091] The receptacles (1, 2), in the preferred embodiment of the invention, are in the form of a revolution, and it is the element in which the nutritive medium is placed for cultivation. The central part (3), in the preferred embodiment of the invention, also has a revolution shape and a diameter slightly greater than the diameter of the receptacles (1, 2).
    [0092]
    [0093] The receptacles (1, 2) comprise an external wall (4) which, in the preferred embodiment of the invention, is circular and smooth on its internal face, and also comprises a perimeter wall (5) that arises perpendicular to the wall external (4).
    [0094]
    [0095] The central part (3) comprises an intermediate wall (6) and two side walls (7) projecting from the perimeter of the intermediate wall (6) in opposite directions. The intermediate wall (6) comprises a first face (8) towards the first receptacle (1) and a second face (9) towards the second receptacle (2).
    [0096]
    [0097] To fix the central piece (3) and the receptacles (1, 2) the central piece (3) can comprise an internal rim (10) on at least one of the faces (8, 9) of the intermediate wall (6) of so that the internal flange (10) and the side wall (7) form a housing (11) for the end of the perimeter wall (5) of a receptacle (1, 2) thus locking both elements.
    [0098]
    [0099] In addition, the intermediate wall (6) houses a hole (13), which is circular in the preferred embodiment of the invention. The diameter of the hole (13) in the preferred embodiment of the invention is approximately one third of the diameter of the intermediate wall (6). However, it is possible that instead of housing a single hole (13) the intermediate wall (6) accommodates more than one hole (13) having other shapes and sizes different from the one proposed in the preferred embodiment, while allowing free exchange gaseous between both receptacles (1, 2) of the culture chamber and limits the possibilities of cross-contamination of the microorganisms in culture.
    [0100]
    [0101] The orifice (13) of the culture chamber object of the invention can be covered by a membrane or a filter (14), which are porous so as to allow the passage of air and volatile compounds, but block the passage of elements of larger size, such as fungal spores. These elements, membrane or filter (14), can be presented industrially attached to the contour of the hole (13), a part or all of any of the faces (8, 9) of the intermediate wall (6) of the piece central or can also be fixed by mechanical means to any of the faces (8, 9) of the intermediate wall (6).
    [0102]
    [0103] In the preferred embodiment of the invention, the dimensions of the perimeter wall (5) of both receptacles (1, 2) are identical, although there are alternative embodiments in which the dimensions of the perimeter wall (5) of each receptacle (1, 2) are different from each other.
    [0104]
    [0105] In the preferred embodiment of the invention, the dimensions of the external wall (4) of the receptacles (1, 2) are identical, although there are alternative embodiments in which the dimensions of the two outer walls (4) are different.
    [0106]
    [0107] In the preferred embodiment of the invention, the dimensions of the two side walls (7) of the central part (3) are identical, although there are alternative embodiments in which the dimensions of the two side walls (7) are different.
    [0108]
    [0109] The dimensions of the intermediate wall (6) of the central part (3) are slightly greater than the dimensions of the external walls (4) of the receptacles (1, 2), whereas, in the preferred embodiment of the invention, the cited walls (4, 6) are circular, the diameter of the intermediate wall (6) is greater than the diameter of the external walls (4). Likewise, in the preferred embodiment of the invention, the perimeter wall (5) of the receptacles (1, 2) has a greater height than the side walls (7) of the intermediate piece.
    [0110]
    [0111] In the culture chamber of the invention, the central piece (3) fits and rests on the second receptacle (2) while allowing the first receptacle (1) to fit and rest on it, so that the perimeter wall (5) ) of each receptacle (1, 2) fits into one of the side walls (7) of the central part (3), furthermore the perimeter walls (5) of the receptacles (1, 2) rest on the intermediate wall (6). ) of the central piece (3), so that the receptacles (1, 2) are facing each other and generate an inner chamber composed of the volume of both receptacles (1, 2) arranged symmetrically and partially communicated through the orifice (13). ) of the intermediate wall (6) of the central piece (3).
    [0112] The fit between the central piece (3) and the receptacles (1, 2) gives stability to the culture chamber as a whole and keeps the components together when the cultivation chamber is not being manipulated.
    [0113]
    [0114] The intermediate wall (6) of the central part (3) is parallel to the external walls (4) of the receptacles (1,2).
    [0115]
    [0116] In the preferred embodiment of the invention, the present culture chamber is made of transparent plastic materials known in the manufacture of other culture plates. However, the culture chamber, in alternative embodiments, is made of glass or any other materials suitable for its function.
    [0117]
    [0118] Although in the preferred embodiment of the invention, the dimensions of both receptacles (1, 2) are identical, have a revolution shape and are shallow, there are alternative embodiments in which the dimensions of the receptacles (1, 2) are not identical , and the shape can vary, since both the receptacles (1, 2) and the central piece (3) can be square, rectangular, etc.
    [0119]
    [0120] In the preferred embodiment of the invention, both the first face (8) and the second face (9) of the intermediate wall (6) of the central part (3) are smooth, and therefore a continuous contact with the end of the head is generated. the perimeter walls (5) of the receptacles (1,2) thus limiting the gaseous exchange with the outside of the culture chamber (non-ventilated plates). However, in alternative embodiments, the first face (8) and / or the second face (9) may have tabs (12) which prevent a continuous contact and which, therefore, form gaps through which a certain result occurs. gaseous exchange with the outside (ventilated plates). There are embodiments in which smooth and expensive faces are combined with tabs (12) so that ventilation can be given to either of the two receptacles (1,2) independently. This gives great versatility to the present invention, allowing to vary the culture conditions and select the most suitable for each specific experiment.
    [0121]
    [0122] Although in the preferred embodiment of the invention, the internal faces of both receptacles (1, 2) are smooth, in alternative embodiments may appear one or more partitions (15) subdividing two or more independent parts inside one or both receptacles (1, 2) allowing several independent cultures in the same receptacle (1, 2).
    [0123] The culture chamber object of the invention comprises stacking means, which allow two or more culture plates to be stacked. Said means of stacking, comprise a first flange (16) located on the external wall (4) of the first receptacle (1) and a second flange (17) of slightly different diameter to the first flange (16) located on the external wall (4) ) of the second receptacle (2), so that facing the external walls (4) of a first receptacle (1) and a second receptacle (2), the first flange (16) fits into the second flange (17) and is prevented the relative movement between two stacked culture plates.
    权利要求:
    Claims (7)
    [1]
    1. Culture chamber for competition microbiological tests using volatile compounds, characterized in that it comprises:
    - a first receptacle (1),
    - a second receptacle (2), and
    - a central piece (3) between the first receptacle (1) and the second receptacle (2), where the receptacles (1, 2) comprise an external wall (4) and a perimeter wall (5) perpendicular to the external wall ( 4), and where the central piece (3) comprises an intermediate wall (6) with at least one hole (13), and two side walls (7) projecting from the perimeter of the intermediate wall (6) in opposite directions , such that the side walls (7) of the central part (3) surround the perimeter walls (5) of the receptacles (1, 2) and the perimeter walls (5) of the receptacles (1, 2) rest on the intermediate wall (6) of the center piece (3).
    [2]
    2. Culture chamber for competing microbiological tests using volatile compounds according to claim 1, characterized in that the intermediate wall (6) of the central part (3) comprises a first face (8) facing the first receptacle (1) and a second face (9) facing the second receptacle (2), where the first face (8) and the second face (9) are smooth.
    [3]
    3. Culture chamber for competition microbiological tests by volatile compounds according to claim 1, characterized in that the intermediate wall (6) of the central part (3) comprises a first face (8) facing the first receptacle (1) and a second face (9) facing the second receptacle (2), wherein at least one of the faces (8, 9) comprises a plurality of tabs (12) where the perimeter wall (5) of a receptacle (1, 2), thus facilitating gas exchange with the exterior. .
    [4]
    4. Culture chamber for competition microbiological tests using volatile compounds according to any of claims 1 to 3, characterized in that the central part (3) comprises an internal rim (10) on at least one of the faces (8, 9) of the intermediate wall (6), such that the internal flange (10) and the side wall (7) form a housing (11) for the end of the perimeter wall (5) of one or both receptacles (1, 2). so nailed.
    [5]
    5. Culture chamber for competition microbiological tests using volatile compounds according to any of claims 1 to 4, characterized in that it comprises a membrane or filter (14) covering the hole (13) of the intermediate wall (6) of the central piece (3), where the membrane or filter (14) is porous allowing the passage of air and volatile compounds, and blocking the passage of larger elements.
    [6]
    6. Culture chamber for competition microbiological tests using volatile compounds according to any of claims 1 to 5, characterized in that it comprises:
    - a first flange (16) located on the external wall (4) of the first receptacle (1), and
    - a second flange (17) located on the external wall (4) of the second receptacle (2),
    where faced the external walls (4) of a first receptacle (1) and a second receptacle (2), the first flange (16) fits into the second flange (17) preventing relative movement between two stacked culture plates.
    [7]
    7. Culture chamber for competing microbiological tests using volatile compounds according to any of claims 1 to 6, characterized in that it comprises at least one partition (15) in at least one receptacle (1, 2), such that the partition (15) ) divides the interior of said receptacle (1, 2) into independent parts.
    类似技术:
    公开号 | 公开日 | 专利标题
    US7771991B2|2010-08-10|Cell cultivating flask
    ES2246064T3|2006-02-01|CULTURE BOX OF MULTIPLE POCILOS.
    JP5107919B2|2012-12-26|Multi-layer cell culture device
    US3597326A|1971-08-03|Multi-dish laboratory unit
    US7078228B2|2006-07-18|Cell cultivating flask
    US10625264B2|2020-04-21|Fitted lid for multi-well plate
    US4673651A|1987-06-16|Multi-cell tray
    FI72999B|1987-04-30|PETRISKAOL.
    ES2646790T3|2017-12-18|Lockable cell growth chamber with anti-lockable feature
    WO1994006902A1|1994-03-31|Plate for cell cultures with a system for laterally diffusing molecules through a barrier membrane
    ES2202406T3|2004-04-01|CULTURE AND ASSEMBLY VESSEL.
    ES2708899A1|2019-04-11|CULTIVATION CHAMBER FOR MICROBIOLOGICAL COMPETITION TESTING THROUGH VOLATILE COMPOUNDS |
    US20140162352A1|2014-06-12|Dual Use Petri Plate With Locking Feature
    US20080078119A1|2008-04-03|Plant starter cell container array rack
    US9783770B2|2017-10-10|Deepwell plate system with lid
    US20040115798A1|2004-06-17|Multiwell plate lid with vents
    KR20140000329U|2014-01-15|Culture vessel structure
    ES1273985U|2021-07-14|CULTIVATION CHAMBER TO EVALUATE THE EFFECTS OF VOLATILE COMPOUNDS ON PLANT-MICROORGANISM INTERACTIONS |
    ES2218123T3|2004-11-16|DEVICE FOR GROWTH AND OBSERVATION OF A BIOLOGICAL CULTURE.
    JP2004337057A|2004-12-02|Laboratory dish
    US8658422B2|2014-02-25|Culture plate comprising a lid for lateral ventilation
    TW202000889A|2020-01-01|Culture container and filter for culture container capable of preventing the invasion of mites and external microorganisms
    CN209508232U|2019-10-18|A kind of multi-compartment microbial cultivation device
    KR101407248B1|2014-06-13|Container for culturing organism
    KR20070119920A|2007-12-21|Vessel for laboratory
    同族专利:
    公开号 | 公开日
    WO2020030835A1|2020-02-13|
    CA3101265A1|2020-02-13|
    US20210198609A1|2021-07-01|
    EP3835408A1|2021-06-16|
    JP2021531735A|2021-11-25|
    ES2708899B2|2019-11-26|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    CN203144398U|2013-03-19|2013-08-21|河南科技学院|Culture dish|
    CN104689861A|2015-02-11|2015-06-10|中国水稻研究所|Biological culture device|
    CN204874519U|2015-07-17|2015-12-16|四川农业大学|Culture dish is used to volatile materials bacteriostatic activity survey|
    法律状态:
    2019-04-11| BA2A| Patent application published|Ref document number: 2708899 Country of ref document: ES Kind code of ref document: A1 Effective date: 20190411 |
    2019-11-26| FG2A| Definitive protection|Ref document number: 2708899 Country of ref document: ES Kind code of ref document: B2 Effective date: 20191126 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201830817A|ES2708899B2|2018-08-10|2018-08-10|CULTURE CAMERA FOR MICROBIOLOGICAL COMPETITION TESTS BY VOLATILE COMPOUNDS|ES201830817A| ES2708899B2|2018-08-10|2018-08-10|CULTURE CAMERA FOR MICROBIOLOGICAL COMPETITION TESTS BY VOLATILE COMPOUNDS|
    US17/058,170| US20210198609A1|2018-08-10|2019-07-05|Culture chamber for microbiological competition assays using volatile compounds|
    JP2020565954A| JP2021531735A|2018-08-10|2019-07-05|Culture chamber for microbial competition assays using volatile compounds|
    EP19846227.7A| EP3835408A1|2018-08-10|2019-07-05|Culture chamber for microbiological competition assays using volatile compounds|
    PCT/ES2019/070475| WO2020030835A1|2018-08-10|2019-07-05|Culture chamber for microbiological competition assays using volatile compounds|
    CA3101265A| CA3101265A1|2018-08-10|2019-07-05|Culture chamber for microbiological competition assays using volatile compounds|
    [返回顶部]